Preparation of stable polymer-liposome complexes by a novel approach employing a one-pot method

The purpose of this study was to prepare stable polymer-liposome complexes using a new, improved one-pot method. Complexes prepared by the conventional method were used as reference. combines vesicle formation and complexation with polymer in single step allowing for fewer steps compared thin-film followed coating. This has possibility complexing on surface within aqueous core liposomes. Soya phosphatidylcholine (SoyPC) combination different amounts either dioleoyl trimethylammoniumpropane (DOTAP, positively charged) or egg phosphatidylglycerol (Egg PG, negatively complexed alginate chitosan, respectively. All alginate-liposome larger naked liposomes zeta potential changed from positive negative after polymer. Polydispersity index (PDI) analysis showed narrow size distributions implying homogenous populations. best stability obtained employing 20 mol% DOTAP membrane. formulation during whole four months period when test performed. chitosan-liposome generally difficult extrude than due need pore membrane extrusion. Based these results, can be preparing complexes. For alternative methods reduction should investigated.